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Image Search Results
Journal: bioRxiv
Article Title: An effective, safe and cost-effective cell-based chimeric vaccine against SARS-CoV2
doi: 10.1101/2020.08.19.258244
Figure Lengend Snippet: (A) The functional domain of SARS-CoV-2 spike protein. (B) Potential B cell antigen of RBD domain from SARS-CoV2 is predicted by Discotope software based on their 3D structure. (C) Potential linear B cell epitopes of SARS-CoV-2 full S protein are analysed with the IEDB database. (D) The location of potential antigens in RBD domain (SARS-CoV-2:red, SARS-CoV: Purple) and interaction model between RBD and ACE2 receptor (interface is marked yellow) are marked with Discovery Studio. (E) The expression of Spike and nucleocapsid with wild-type sequence in 293T cells are detected by Western Blot assay. (F) The expression of Spike and its derivatives with codon optimization (opt).
Article Snippet: The coding sequence of Spike, Nucleocapsid derived from SARS-COV2 (MN908947.3), and human IgG Fc fragment (BC065820.1) was synthesized by Sangon Biotech,
Techniques: Functional Assay, Software, Expressing, Sequencing, Western Blot
Journal: bioRxiv
Article Title: An effective, safe and cost-effective cell-based chimeric vaccine against SARS-CoV2
doi: 10.1101/2020.08.19.258244
Figure Lengend Snippet: (A) Potential B-cell epitopes of N protein is predicted by IEDB database. (B) Potential MHCI-binding peptides of N. (C) Functional domain of SARS-CoV N protein (Upper) and its antibody epitope map reported in previous study. (D) The skeleton of Chimeric Vaccine for SARS-CoV-2, RBD: spike RBD domain (306-541 aa), Ntap: T-cell-associated peptide of N (211-339 aa). (E) Characterization of SARS-CoV-2-derived protein and C-Vac antigen by SARS-CoV-2 antisera and commercial antibodies against SARS-CoV2 spike RBD or Nucleocapsid.
Article Snippet: The coding sequence of Spike, Nucleocapsid derived from SARS-COV2 (MN908947.3), and human IgG Fc fragment (BC065820.1) was synthesized by Sangon Biotech,
Techniques: Binding Assay, Functional Assay, Derivative Assay